Phytophthora species recovered from the Connecticut River Valley in Massachusetts, USA.
Identifieur interne : 001566 ( Main/Exploration ); précédent : 001565; suivant : 001567Phytophthora species recovered from the Connecticut River Valley in Massachusetts, USA.
Auteurs : Nicholas J. Brazee [États-Unis] ; Robert L. Wick [États-Unis] ; Jonathan P. Hulvey [États-Unis]Source :
- Mycologia [ 0027-5514 ]
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Complexe IV de la chaîne respiratoire (génétique), Données de séquences moléculaires (MeSH), Espaceur de l'ADN ribosomique (composition chimique), Espaceur de l'ADN ribosomique (génétique), Forêts (MeSH), Magnoliopsida (MeSH), Massachusetts (MeSH), Phylogenèse (MeSH), Phytophthora (classification), Phytophthora (génétique), Rivières (MeSH), Séquence nucléotidique (MeSH), Tubuline (génétique), Variation génétique (MeSH).
- MESH :
- composition chimique : Espaceur de l'ADN ribosomique, Phytophthora.
- génétique : Complexe IV de la chaîne respiratoire, Espaceur de l'ADN ribosomique, Phytophthora, Tubuline.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Forêts, Magnoliopsida, Massachusetts, Phylogenèse, Rivières, Séquence nucléotidique, Variation génétique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), DNA, Ribosomal Spacer (chemistry), DNA, Ribosomal Spacer (genetics), Electron Transport Complex IV (genetics), Forests (MeSH), Genetic Variation (MeSH), Magnoliopsida (MeSH), Massachusetts (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Phytophthora (classification), Phytophthora (genetics), Rivers (MeSH), Sequence Analysis, DNA (MeSH), Tubulin (genetics).
- MESH :
- chemical , chemistry : DNA, Ribosomal Spacer.
- chemical , genetics : DNA, Ribosomal Spacer, Electron Transport Complex IV, Tubulin.
- classification : Phytophthora.
- genetics : Phytophthora.
- Base Sequence, Forests, Genetic Variation, Magnoliopsida, Massachusetts, Molecular Sequence Data, Phylogeny, Rivers, Sequence Analysis, DNA.
Abstract
Little is currently known about the assemblage of Phytophthora species in northeastern North America, representing a gap in our understanding of species incidence. Therefore, Phytophthora species were surveyed at 20 sites in Massachusetts, with 16 occurring in the Connecticut River Valley. Many of the sampled waterways were adjacent to active agricultural lands, yet were buffered by mature floodplain forests composed of Acer, Platanus, Populus and Ulmus. Isolates were recovered with three types of baits (rhododendron leaves, pear, green pepper) in 2013 and water filtration in 2014. Overall, 457 isolates of Phytophthora were recovered and based on morphological characters and rDNA internal transcribed spacer (ITS), β-tubulin (β-tub) and cytochrome oxidase c subunit I (cox1) sequences, 18 taxa were identified, including three new species: P. taxon intercalaris, P. taxon caryae and P. taxon pocumtuck. In addition, 49 isolates representing five species of Phytopythium also were identified. Water filtration captured a greater number of taxa (18) compared to leaf and fruit baits (12). Of the three bait types rhododendron leaves yielded the greatest number of isolates and taxa, followed by pear and green pepper, respectively. Despite the proximity to agricultural lands, none of the Phytophthora species baited are considered serious pathogens of vegetable crops in the region. However, many of the recovered species are known woody plant pathogens, including four species in the P. citricola s.l. complex that were identified: P. plurivora, P. citricola III, P. pini and a putative novel species, referred to here as P. taxon caryae. An additional novel species, P. taxon pocumtuck, is a close relative of P. borealis based on cox1 sequences. The results illustrate a high level of Phytophthora species richness in the Connecticut River Valley and that major rivers can serve as a source of inoculum for pathogenic Phytophthora species in the northeast.
DOI: 10.3852/15-038
PubMed: 26553775
Affiliations:
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Brazee</name><affiliation wicri:level="4"><nlm:affiliation>UMass Extension, Center for Agriculture, Food and the Environment, University of Massachusetts, Amherst, Massachusetts 01002 nbrazee@umass.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>UMass Extension, Center for Agriculture, Food and the Environment, University of Massachusetts, Amherst</wicri:regionArea><orgName type="university">Université du Massachusetts</orgName><placeName><settlement type="city">Amherst (Massachusetts)</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Wick, Robert L" sort="Wick, Robert L" uniqKey="Wick R" first="Robert L" last="Wick">Robert L. 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Brazee</name><affiliation wicri:level="4"><nlm:affiliation>UMass Extension, Center for Agriculture, Food and the Environment, University of Massachusetts, Amherst, Massachusetts 01002 nbrazee@umass.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>UMass Extension, Center for Agriculture, Food and the Environment, University of Massachusetts, Amherst</wicri:regionArea><orgName type="university">Université du Massachusetts</orgName><placeName><settlement type="city">Amherst (Massachusetts)</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Wick, Robert L" sort="Wick, Robert L" uniqKey="Wick R" first="Robert L" last="Wick">Robert L. 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Hulvey</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Department of Biology, University of Massachusetts, Amherst</wicri:cityArea></affiliation></author></analytic><series><title level="j">Mycologia</title><idno type="ISSN">0027-5514</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>DNA, Ribosomal Spacer (chemistry)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Electron Transport Complex IV (genetics)</term><term>Forests (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Magnoliopsida (MeSH)</term><term>Massachusetts (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytophthora (classification)</term><term>Phytophthora (genetics)</term><term>Rivers (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Tubulin (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Complexe IV de la chaîne respiratoire (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Espaceur de l'ADN ribosomique (composition chimique)</term><term>Espaceur de l'ADN ribosomique (génétique)</term><term>Forêts (MeSH)</term><term>Magnoliopsida (MeSH)</term><term>Massachusetts (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phytophthora (classification)</term><term>Phytophthora (génétique)</term><term>Rivières (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Tubuline (génétique)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal Spacer</term><term>Electron Transport Complex IV</term><term>Tubulin</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Espaceur de l'ADN ribosomique</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Complexe IV de la chaîne respiratoire</term><term>Espaceur de l'ADN ribosomique</term><term>Phytophthora</term><term>Tubuline</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Forests</term><term>Genetic Variation</term><term>Magnoliopsida</term><term>Massachusetts</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Rivers</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Forêts</term><term>Magnoliopsida</term><term>Massachusetts</term><term>Phylogenèse</term><term>Rivières</term><term>Séquence nucléotidique</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Little is currently known about the assemblage of Phytophthora species in northeastern North America, representing a gap in our understanding of species incidence. Therefore, Phytophthora species were surveyed at 20 sites in Massachusetts, with 16 occurring in the Connecticut River Valley. Many of the sampled waterways were adjacent to active agricultural lands, yet were buffered by mature floodplain forests composed of Acer, Platanus, Populus and Ulmus. Isolates were recovered with three types of baits (rhododendron leaves, pear, green pepper) in 2013 and water filtration in 2014. Overall, 457 isolates of Phytophthora were recovered and based on morphological characters and rDNA internal transcribed spacer (ITS), β-tubulin (β-tub) and cytochrome oxidase c subunit I (cox1) sequences, 18 taxa were identified, including three new species: P. taxon intercalaris, P. taxon caryae and P. taxon pocumtuck. In addition, 49 isolates representing five species of Phytopythium also were identified. Water filtration captured a greater number of taxa (18) compared to leaf and fruit baits (12). Of the three bait types rhododendron leaves yielded the greatest number of isolates and taxa, followed by pear and green pepper, respectively. Despite the proximity to agricultural lands, none of the Phytophthora species baited are considered serious pathogens of vegetable crops in the region. However, many of the recovered species are known woody plant pathogens, including four species in the P. citricola s.l. complex that were identified: P. plurivora, P. citricola III, P. pini and a putative novel species, referred to here as P. taxon caryae. An additional novel species, P. taxon pocumtuck, is a close relative of P. borealis based on cox1 sequences. The results illustrate a high level of Phytophthora species richness in the Connecticut River Valley and that major rivers can serve as a source of inoculum for pathogenic Phytophthora species in the northeast. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26553775</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>05</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0027-5514</ISSN><JournalIssue CitedMedium="Print"><Volume>108</Volume><Issue>1</Issue><PubDate><MedlineDate>2016 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Mycologia</Title><ISOAbbreviation>Mycologia</ISOAbbreviation></Journal><ArticleTitle>Phytophthora species recovered from the Connecticut River Valley in Massachusetts, USA.</ArticleTitle><Pagination><MedlinePgn>6-19</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3852/15-038</ELocationID><Abstract><AbstractText>Little is currently known about the assemblage of Phytophthora species in northeastern North America, representing a gap in our understanding of species incidence. Therefore, Phytophthora species were surveyed at 20 sites in Massachusetts, with 16 occurring in the Connecticut River Valley. Many of the sampled waterways were adjacent to active agricultural lands, yet were buffered by mature floodplain forests composed of Acer, Platanus, Populus and Ulmus. Isolates were recovered with three types of baits (rhododendron leaves, pear, green pepper) in 2013 and water filtration in 2014. Overall, 457 isolates of Phytophthora were recovered and based on morphological characters and rDNA internal transcribed spacer (ITS), β-tubulin (β-tub) and cytochrome oxidase c subunit I (cox1) sequences, 18 taxa were identified, including three new species: P. taxon intercalaris, P. taxon caryae and P. taxon pocumtuck. In addition, 49 isolates representing five species of Phytopythium also were identified. Water filtration captured a greater number of taxa (18) compared to leaf and fruit baits (12). Of the three bait types rhododendron leaves yielded the greatest number of isolates and taxa, followed by pear and green pepper, respectively. Despite the proximity to agricultural lands, none of the Phytophthora species baited are considered serious pathogens of vegetable crops in the region. However, many of the recovered species are known woody plant pathogens, including four species in the P. citricola s.l. complex that were identified: P. plurivora, P. citricola III, P. pini and a putative novel species, referred to here as P. taxon caryae. An additional novel species, P. taxon pocumtuck, is a close relative of P. borealis based on cox1 sequences. The results illustrate a high level of Phytophthora species richness in the Connecticut River Valley and that major rivers can serve as a source of inoculum for pathogenic Phytophthora species in the northeast. </AbstractText><CopyrightInformation>© 2016 by The Mycological Society of America.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Brazee</LastName><ForeName>Nicholas J</ForeName><Initials>NJ</Initials><AffiliationInfo><Affiliation>UMass Extension, Center for Agriculture, Food and the Environment, University of Massachusetts, Amherst, Massachusetts 01002 nbrazee@umass.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wick</LastName><ForeName>Robert L</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Stockbridge School of Agriculture, University of Massachusetts, Amherst, Massachusetts 01003.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hulvey</LastName><ForeName>Jonathan P</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>11</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mycologia</MedlineTA><NlmUniqueID>0400764</NlmUniqueID><ISSNLinking>0027-5514</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014404">Tubulin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.9.3.1</RegistryNumber><NameOfSubstance UI="D003576">Electron Transport Complex IV</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003576" MajorTopicYN="N">Electron Transport Complex IV</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D065928" MajorTopicYN="N">Forests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019684" MajorTopicYN="N">Magnoliopsida</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008404" MajorTopicYN="N">Massachusetts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045483" MajorTopicYN="N">Rivers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014404" MajorTopicYN="N">Tubulin</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">cytochrome oxidase</Keyword><Keyword MajorTopicYN="N">floodplain forests</Keyword><Keyword MajorTopicYN="N">oomycetes</Keyword><Keyword MajorTopicYN="N">β-tubulin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>02</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>10</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26553775</ArticleId><ArticleId IdType="pii">15-038</ArticleId><ArticleId IdType="doi">10.3852/15-038</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region><settlement><li>Amherst (Massachusetts)</li></settlement><orgName><li>Université du Massachusetts</li></orgName></list><tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Brazee, Nicholas J" sort="Brazee, Nicholas J" uniqKey="Brazee N" first="Nicholas J" last="Brazee">Nicholas J. Brazee</name></region><name sortKey="Hulvey, Jonathan P" sort="Hulvey, Jonathan P" uniqKey="Hulvey J" first="Jonathan P" last="Hulvey">Jonathan P. Hulvey</name><name sortKey="Wick, Robert L" sort="Wick, Robert L" uniqKey="Wick R" first="Robert L" last="Wick">Robert L. Wick</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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